FIG. 1.

Schematic overview of bsh mutants made in L. plantarum WCFS1 and PCR analysis encompassing the bsh locus of the mutants. Sizes of PCR products are indicated. For the wild-type locus of bsh1, bsh2, bsh3, and bsh4, the sizes of the PCR products were 1.4, 1.4, 1.4, and 1.2 kb, respectively, whereas for the mutated locus of bsh1, bsh2, bsh3, and bsh4, the sizes of the PCR products were 0.5, 0.4, 0.5, and 0.3 kb, respectively. Δbsh1,2,3,4, NZ5332; Δbsh1, NZ5305; Δbsh2, NZ5307; Δbsh3, NZ5309; Δbsh4, NZ5311; Δbsh2,3,4, NZ5330; Δbsh1,3,4, NZ5328; Δbsh1,2,4, NZ5326; Δbsh1,2,3, NZ5324.