TABLE 2.
Detection of virulence genes by PCR
| Target gene (phenotype) | Accession no. | Primer | Nucleotide sequence (5′→3′) | PCR conditions | No. of PCR cycles | PCR product (bp) | Reference |
|---|---|---|---|---|---|---|---|
| stx2e (Stx2e, B subunit) | M21534 | SLT-IIBv1 | ATGAAGAAGATGTTTATAGCG | 94.0°C, 30 s; 62.0°C, 60 s; 72.0°C, 30 s | 30 | 261 | 2 |
| SLT-IIBv2 | GTTAAACTTCACCTGGGCAAAG | ||||||
| astA (EAST1) | L11241 | EAST11a | CCATCAACACAGTATATCCGA | 95°C, 30 s; 55°C, 120 s; 72°C, 120 | 30 | 111 | 52 |
| EAST11b | GGTCGCGAGTGACGGCTTTGT | ||||||
| STII (STb) | M35586 | STb-f | CGCATTTCTTCTTGCATCTATG | 94°C, 40 s; 55°C, 40 s; 72°C, 40 s | 30 | 189 | This work |
| STb-r | TGCTGCAACCATTATTTGGG | ||||||
| STI-h (human) | M34916 | STIh-f | TCCCTCAGGATGCTAAAC | 94°C, 40 s; 47°C, 60 s; 72°C, 40 s | 30 | 244 | 6 |
| ST1h-b | GCAACAGGTACATACGTT | ||||||
| STI-p (animal) | M25607 | STIp-f | TTCTGTATTATCTTTCCCC | 94°C, 40 s; 51.3°C, 40 s; 72°C, 40 s | 30 | 258 | 6 |
| ST1p-b | TTATGATTTTCTCAGCACC | ||||||
| faeG (F4 [K88]) | AJ616236 | K88-f | GGTGATTTCAATGGTTCGGTC | 94°C, 30 s; 61.8°C 60 s; 72°C 45 s | 30 | 766 | This work |
| K88-r | ATTGCTACGTTCAGCGGAGCG | ||||||
| fan (F5 [K99], fimbrial subunit) | M35282 | K99-f | GACTACCAATGCTTCTGCGA | 95.0°C, 30 s; 60.0°C 45 s; 72.0°C 45 s | 30 | 459 | This work |
| K99-b | GGTGGATATAAAGCTGGCGT | ||||||
| fasA (F6 [987p], fimbrial subunit) | M35257 | 987p-f | CTGCCAGTCTATGCCAAGTG | 94°C, 30 s; 61.8°C, 60 s; 72°C, 45 s | 30 | 459 | This work |
| 987p-b | ACGGTGTACCTGCTGAACGAATAG | ||||||
| fedA (F18 [F107], fimbrial subunit) | M61713 | F107-f | CTTTCACATTGCGTGTGGAG | 94°C, 30 s; 61.8°C, 60 s; 72°C, 45 s | 30 | 513 | This work |
| F107-r | ACCACCTTTCAGTTGAGCAG | ||||||
| Fim41a (F41, fimbrial subunit) | X14354 | F41-f | GCATCAGCGGCAGTATCT | 95.0°C, 30 s; 60.0°C 45 s; 72.0°C 45 s | 30 | 380 | 14 |
| F41-b | AGGTGATAATACTGAGCTAGGGAC |