TABLE 5.
No. of P27 gene loci | PCR detection of bacteriophage P27 genes outside stxAB2ea
|
No. of positive strains | |||
---|---|---|---|---|---|
Integrase (yecE-int [L01]) (0-283)b | Putative P27 Q gene (L21) (13854-14056) | DNA methylase- stxA2e (L24) (16828-17599) | Holin (stxB2e-S [L28]) (18607-19405) | ||
0 | 0 | 0 | 0 | 0 | 1 |
1 | + | 0 | 0 | 0 | 6 |
1 | 0 | 0 | + | 0 | 46 |
2 | 0 | 0 | + | + | 23 |
2 | + | 0 | 0 | + | 8 |
3 | + | 0 | + | + | 6 |
3 | + | + | + | 0 | 13 |
4 | + | + | + | + | 7 |
No. of positive strains | 40 | 20 | 95 | 44 |
0, PCR negative; +, PCR positive.
PCR product position in bacteriophage P27 sequence (accession no. AJ298298).