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. 2008 Jun 6;74(15):4923–4933. doi: 10.1128/AEM.00470-08

FIG. 4.

FIG. 4.

Determination of specific β-Gal activity for quantification of pksPp-lacZ expression. (A) The pksPp-lacZ strain, bearing a pksP promoter-lacZ gene fusion, and the pksPp-lacZ ΔpkaR mutant, with a deletion of the pkaR gene, were cultivated for 28 h in AMM. Protein extracts were analyzed for β-Gal activity. (B) The pksPp-lacZ and pksPp-lacZ-pkaC1Oex strains were precultivated for 16 h in AMM. The mycelia were then shifted to acetate-containing medium and further incubated for 8 h for induction of pkaC1 overexpression. Protein extracts were analyzed for β-Gal activity.