TABLE 2.
Putative function and spot no.a | Protein name | Locus tag | % Sequence coverage | Regulation (fold) |
---|---|---|---|---|
Protein biosynthesis | ||||
403 | Translation elongation factor EF-2 subunit, putative | AFUA_2G13530 | 13.5 | 3.7 |
441 | Translation elongation factor EF-2 subunit, putative | AFUA_2G13530 | 19.4 | 5.6 |
418 | Polyadenylate-binding protein | AFUA_1G04190 | 50.5 | 2.4 |
852 | 40S ribosomal protein S0 | AFUA_3G13320 | 50.5 | 8.1 |
Purine biosynthesis | ||||
426 | Ade1p protein | AFUA_6G04730 | 27.5 | 2.3 |
Pyrimidine/arginine biosynthesis | ||||
275 | Carbamoyl-phosphate synthetase, large subunit | AFUA_2G10070 | 18.2 | 3.2 |
Tricarboxylic acid cycle | ||||
1007 | Malate dehydrogenase, NAD dependent | AFUA_6G05210 | 71.5 | 3.0 |
Glyoxylate cycle | ||||
565 | Malate synthase | AFUA_6G03540 | 32.4 | 2.4 |
628 | Malate synthase | AFUA_6G03540 | 25.4 | 2.7 |
634 | Malate synthase | AFUA_6G03540 | 37.9 | 2.7 |
2100 | Isocitrate lyase (AcuD) | AFUA_6G02860 | 17.4 | 2.4 |
Gluconeogenesis | ||||
619 | Phosphoenolpyruvate carboxykinase (AcuF) | AFUA_6G07720 | 28.3 | 2.8 |
2203 | Phosphoenolpyruvate carboxykinase (AcuF) | AFUA_6G07720 | 34.6 | 2.5 |
Pentose phosphate pathway | ||||
1039 | Ribose 5-phosphate isomerase A | AFUA_6G10610 | 18.4 | 2.1 |
Acetate degradation | ||||
501 | Acetyl-CoA synthetase (FacA) | AFUA_4G11080 | 16.0 | 3.5 |
510 | Acetyl-CoA synthetase (FacA) | AFUA_4G11080 | 18.2 | 11.0 |
518 | Acetyl-CoA synthetase (FacA) | AFUA_4G11080 | 26.7 | 2.8 |
519 | Acetyl-CoA synthetase (FacA) | AFUA_4G11080 | 29.7 | 4.8 |
2266 | Acetyl-CoA synthetase (FacA) | AFUA_4G11080 | 30.4 | 7.6 |
Alcohol degradation | ||||
891 | Alcohol dehydrogenase (ADH1) | AFUA_7G01010 | 37.7 | 2.8 |
914 | Alcohol dehydrogenase (ADH1) | AFUA_7G01010 | 33.7 | 2.6 |
Fatty acid degradation | ||||
791 | 3-Ketoacyl-CoA ketothiolase (Kat1), putative | AFUA_1G12650 | 45.5 | 3.0 |
Propionate degradation | ||||
771 | Methylcitrate synthase | AFUA_6G03590 | 40.0 | 2.4 |
Electron transport and reduction | ||||
835 | Flavohemoprotein | AFUA_8G06080 | 22.9 | 2.2 |
2179 | Assimilatory sulfite reductase | AFUA_6G08920 | 17.0 | 2.2 |
Cytoskeleton assembly | ||||
701 | Tubulin beta-2 subunit | AFUA_7G00250 | 22.6 | 3.2 |
710 | Tubulin beta-2 subunit | AFUA_7G00250 | 23.3 | 2.0 |
780 | Septin | AFUA_5G03080 | 21.6 | 2.7 |
Protection against oxidative stress | ||||
464 | Mycelial catalase Cat1 | AFUA_3G02270 | 34.6 | 2.1 |
466 | Mycelial catalase Cat1 | AFUA_3G02270 | 37.8 | 5.8 |
Siderophore biosynthesis | ||||
656 | l-Ornithine N-5-oxygenase | AFUA_2G07680 | 45.5 | 2.7 |
Hemolysis | ||||
1457 | Asp hemolysin | AFUA_3G00590 | 50.4 | 2.4 |
Others and proteins of unknown function | ||||
597 | GMC oxidoreductase | AFUA_3G01580 | 49.1 | 2.4 |
603 | GMC oxidoreductase | AFUA_3G01580 | 25.9 | 8.0 |
610 | GMC oxidoreductase | AFUA_3G01580 | 41.3 | 2.8 |
545 | AMP-binding domain protein, putative | AFUA_1G05980 | 38.3 | 3.6 |
593 | Pyruvate decarboxylase PdcA, putative | AFUA_3G11070 | 37.1 | 2.1 |
611 | Pyridine nucleotide-disulfide oxidoreductase (Nfrl), putative | AFUA_7G02070 | 37.1 | 3.2 |
719 | Homocitrate synthase | AFUA_4G10460 | 22.2 | 2.1 |
739 | Gamma-butyrobetaine hydroxylase subfamily, putative | AFUA_2G14970 | 43.3 | 2.6 |
776 | Acyl-CoA dehydrogenase family protein | AFUA_5G06500 | 24.6 | 2.2 |
834 | 2-Amino-3-carboxymuconate-6-semialdehyde decarboxylase, putative | AFUA_5G12460 | 27.9 | 2.0 |
903 | Ran/Spi1 binding protein | AFUA_5G12180 | 45.7 | 2.5 |
887 | Unknown function | AFUA_6G11850 | 57.4 | 2.4 |
1019 | Short chain dehydrogenase, putative | AFUA_4G08710 | 59.6 | 3.6 |
1021 | Unknown function | AFUA_8G00550 | 63.1 | 7.9 |
1103 | Outer mitochondrial membrane protein porin | AFUA_4G06910 | 33.0 | 2.4 |
1141 | DlpA domain protein | AFUA_4G10940 | 29.4 | 2.1 |
1165 | Unknown function | AFUA_5G14680 | 63.0 | 2.1 |
1195 | Rho-GDP dissociation inhibitor | AFUA_5G11380 | 29.4 | 2.2 |
1320 | HEX1 | AFUA_5G08830 | 30.3 | 2.3 |
2176 | PH domain protein | AFUA_4G12450 | 20.9 | 8.3 |
Some spots represent different isoforms of the same protein. Proteins were identified by peptide mass fingerprinting. Spot numbers are shown in Fig. 6.