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. 2008 Jun 6;74(15):4923–4933. doi: 10.1128/AEM.00470-08

TABLE 2.

Downregulated proteins of A. fumigatus pkaC1Oex strain in comparison to the wild type after induction of pkaC1 expression

Putative function and spot no.a Protein name Locus tag % Sequence coverage Regulation (fold)
Protein biosynthesis
    403 Translation elongation factor EF-2 subunit, putative AFUA_2G13530 13.5 3.7
    441 Translation elongation factor EF-2 subunit, putative AFUA_2G13530 19.4 5.6
    418 Polyadenylate-binding protein AFUA_1G04190 50.5 2.4
    852 40S ribosomal protein S0 AFUA_3G13320 50.5 8.1
Purine biosynthesis
    426 Ade1p protein AFUA_6G04730 27.5 2.3
Pyrimidine/arginine biosynthesis
    275 Carbamoyl-phosphate synthetase, large subunit AFUA_2G10070 18.2 3.2
Tricarboxylic acid cycle
    1007 Malate dehydrogenase, NAD dependent AFUA_6G05210 71.5 3.0
Glyoxylate cycle
    565 Malate synthase AFUA_6G03540 32.4 2.4
    628 Malate synthase AFUA_6G03540 25.4 2.7
    634 Malate synthase AFUA_6G03540 37.9 2.7
    2100 Isocitrate lyase (AcuD) AFUA_6G02860 17.4 2.4
Gluconeogenesis
    619 Phosphoenolpyruvate carboxykinase (AcuF) AFUA_6G07720 28.3 2.8
    2203 Phosphoenolpyruvate carboxykinase (AcuF) AFUA_6G07720 34.6 2.5
Pentose phosphate pathway
    1039 Ribose 5-phosphate isomerase A AFUA_6G10610 18.4 2.1
Acetate degradation
    501 Acetyl-CoA synthetase (FacA) AFUA_4G11080 16.0 3.5
    510 Acetyl-CoA synthetase (FacA) AFUA_4G11080 18.2 11.0
    518 Acetyl-CoA synthetase (FacA) AFUA_4G11080 26.7 2.8
    519 Acetyl-CoA synthetase (FacA) AFUA_4G11080 29.7 4.8
    2266 Acetyl-CoA synthetase (FacA) AFUA_4G11080 30.4 7.6
Alcohol degradation
    891 Alcohol dehydrogenase (ADH1) AFUA_7G01010 37.7 2.8
    914 Alcohol dehydrogenase (ADH1) AFUA_7G01010 33.7 2.6
Fatty acid degradation
    791 3-Ketoacyl-CoA ketothiolase (Kat1), putative AFUA_1G12650 45.5 3.0
Propionate degradation
    771 Methylcitrate synthase AFUA_6G03590 40.0 2.4
Electron transport and reduction
    835 Flavohemoprotein AFUA_8G06080 22.9 2.2
    2179 Assimilatory sulfite reductase AFUA_6G08920 17.0 2.2
Cytoskeleton assembly
    701 Tubulin beta-2 subunit AFUA_7G00250 22.6 3.2
    710 Tubulin beta-2 subunit AFUA_7G00250 23.3 2.0
    780 Septin AFUA_5G03080 21.6 2.7
Protection against oxidative stress
    464 Mycelial catalase Cat1 AFUA_3G02270 34.6 2.1
    466 Mycelial catalase Cat1 AFUA_3G02270 37.8 5.8
Siderophore biosynthesis
    656 l-Ornithine N-5-oxygenase AFUA_2G07680 45.5 2.7
Hemolysis
    1457 Asp hemolysin AFUA_3G00590 50.4 2.4
Others and proteins of unknown function
    597 GMC oxidoreductase AFUA_3G01580 49.1 2.4
    603 GMC oxidoreductase AFUA_3G01580 25.9 8.0
    610 GMC oxidoreductase AFUA_3G01580 41.3 2.8
    545 AMP-binding domain protein, putative AFUA_1G05980 38.3 3.6
    593 Pyruvate decarboxylase PdcA, putative AFUA_3G11070 37.1 2.1
    611 Pyridine nucleotide-disulfide oxidoreductase (Nfrl), putative AFUA_7G02070 37.1 3.2
    719 Homocitrate synthase AFUA_4G10460 22.2 2.1
    739 Gamma-butyrobetaine hydroxylase subfamily, putative AFUA_2G14970 43.3 2.6
    776 Acyl-CoA dehydrogenase family protein AFUA_5G06500 24.6 2.2
    834 2-Amino-3-carboxymuconate-6-semialdehyde decarboxylase, putative AFUA_5G12460 27.9 2.0
    903 Ran/Spi1 binding protein AFUA_5G12180 45.7 2.5
    887 Unknown function AFUA_6G11850 57.4 2.4
    1019 Short chain dehydrogenase, putative AFUA_4G08710 59.6 3.6
    1021 Unknown function AFUA_8G00550 63.1 7.9
    1103 Outer mitochondrial membrane protein porin AFUA_4G06910 33.0 2.4
    1141 DlpA domain protein AFUA_4G10940 29.4 2.1
    1165 Unknown function AFUA_5G14680 63.0 2.1
    1195 Rho-GDP dissociation inhibitor AFUA_5G11380 29.4 2.2
    1320 HEX1 AFUA_5G08830 30.3 2.3
    2176 PH domain protein AFUA_4G12450 20.9 8.3
a

Some spots represent different isoforms of the same protein. Proteins were identified by peptide mass fingerprinting. Spot numbers are shown in Fig. 6.