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Analysis of the PF proteins in the mutants. (a) Western blot analysis of the purified PFs from wild type and four double mutants. (b and c) SDS-PAGE (b) and Western blot (c) analysis of the purified PFs from the wild type and the flaB1 flaB2 mutant. Rabbit antisera to FlaA, and monoclonal antibodies to FlaB were used as probes.
