FIG. 6.
Fate of GerD-FLAG following spore germination. Spores of strain PP19 (gerD amyE::PsspB-gerD-flag) were incubated in LB medium plus 10 mM l-alanine for 0 (lane 1), 0.75 (lane 2), 1 (lane 3), 2 (lane 4), 2.5 (lane 5), and 3 h (lane 6), and any dormant spores were removed as described in Materials and Methods. The purified germinated spores and/or cells were lyophilized, disrupted, and extracted, aliquots of total extracts from equal amounts of starting dormant spores were run by SDS-PAGE, proteins were transferred to a PVDF membrane, and GerD-FLAG was detected with anti-FLAG antibody. The numbered lines to the left of the figure present the migration positions of molecular mass markers, in kDa, and the arrowheads to the right indicate the predicted migration positions of GerD-FLAG dimers (D) and trimers (T).