TABLE 2.
Germination of spores with GerD-FLAGa
| Strain | Genotype | Rate of spore germination (%/h) with:
|
|
|---|---|---|---|
| l-Alanine | AGFK | ||
| PS832 | Wild type | 84 | 61 |
| FB62 | gerD | <5 | <5 |
| PP17 | gerD amyE::gerD-flag | 79 | 59 |
| PP19 | gerD amyE::PsspB-gerD-flag | 78 | 56 |
| PP21 | gerD lgt amyE::gerD-flag | <5 | 10 |
| PP23 | gerD lgt amyE::PsspB-gerD-flag | <5 | 13 |
a
Spore germination was initiated by addition of either l-alanine or AGFK, germination was monitored by measuring the OD600 of cultures, and rates of germination were calculated as described in Materials and Methods.