TABLE 2.
Dispensability analysis of S. aureus tarK and tarL genes
| S. aureus integrant strain genotyped | Complementing plasmidd | Phenotype (no. of colonies)
|
||
|---|---|---|---|---|
| Wild type | Nonexcisant | Mutant | ||
| tarK (SACOL0238) | 49 | 0 | 55 | |
| tarL (SACOL0242)a | 85 | 0 | 53 | |
| tarK ΔtarL* | 104 | 0 | 0 | |
| pLI50-tarK | 52 | 2 | 50 | |
| pLI50-tarL | 68 | 0 | 36 | |
| pG164-tarKLb | 66 | 1 | 37 | |
| pG164-W23_tarKc* | 104 | 0 | 0 | |
| pG164-W23_tarLc* | 104 | 0 | 0 | |
| pG164-W23_tarKLc | 76 | 0 | 28 | |
a
Total of 138 colonies selected.
b
pG164 induced with 0.4 mM IPTG.
c
pG164 induced with 1.0 mM IPTG.
d
*, statistically significant (P < 0.05).