TABLE 3.
Comparison of CDP-ribitol synthase activities of different loci
Activity, enzyme, and substrate | Km (μM) | kcat (s−1) | kcat/Km (M−1/s−1) |
---|---|---|---|
Reductase activity | |||
TarI′J′ | |||
NADPH | 6.21 ± 1.07 | 0.41 ± 0.01 | 6.60 × 104 |
Ribulose-5-P | 11.0 ± 0.9 | 3.73 × 104 | |
TarIJa | |||
NADPH | 6.74 ± 1.64 | 0.79 ± 0.06 | 1.17 × 105 |
Ribulose-5-P | 28.5 ± 13.1 | 2.77 × 104 | |
Cytidylyltransferase activity | |||
TarI′J′ | |||
CTP | 38.7 ± 6.20 | 66.7 ± 1.4 | 1.72 × 106 |
Ribitol-5-P | 102.2 ± 9.6 | 6.53 × 105 | |
TarIJa | |||
CTP | 78.9 ± 33.2 | 76.8 ± 6.5 | 9.73 × 105 |
Ribitol-5-P | 1,280 ± 390 | 5.98 × 104 |
Data for TarIJ were previously published by Pereira et al. (26).