TABLE 2.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| pLT06 | Integration vector, Cmr derivative of pCJK47 | L. Thurlow, unpublished data |
| pVT01 | pLT06 containing a 2.0-kb EcoRI/BamHI fragment containing engineered gelE deletion | This study |
| pVT02 | pLT06 containing a 2.0-kb EcoRI/BamHI fragment containing engineered sprE deletion | This study |
| pVT03 | pLT06 containing a 2.0-kb EcoRI/BamHI fragment containing engineered gelE sprE deletion | This study |
| pAT28 | Broad-host-range shuttle vector, spectinomycin resistance | 53 |
| pVT05 | pAT28 containing 1748-bp EcoRI/XhoI fragment containing the native gelE promoter along with full-length gelE | This study |
| pVT07 | pAT28 containing 2687-bp EcoRI/BamHI fragment containing the native gelE promoter and full-length gelE sprE | This study |
| pVT08 | pAT28 containing 2123-bp EcoRI/BamHI fragment containing the native gelE promoter, a truncated gelE, and full-length sprE | This study |
| pMV158gfp | Gram-positive replicative vector expressing a Gfp reporter | 1 |