TABLE 3.
Oligonucleotides used in this study
| Primer | Sequence (5′-3′) |
|---|---|
| GelEP1 | GAGAGAATTCGATTGGCTTAGTCATTGAAGC T |
| GelEP2 | CTCTCTCGAGAAAGATGCCTGTACCTAAAATG |
| GelEP3 | GAGAGTCGACCAGGTAAACCAACCAAGTGAAT |
| GelEP4 | CTCTGGATCCCCGTGATTCTGGAAATTCCGAG |
| SprEP1 | GAGAGAATTCGTGAACGCTACAGATGGAACAA |
| SprEP2 | CTCTCTCGAGTTCATTCATTGACCAGAACAGA |
| SprEP3 | GAGAGTCGACCTCGGAATTTCCAGAATCACGG |
| SprEP4 | CTCTGGATCCAGGTTCAGCGTTATCTACTAAG |
| GelEUp | CGCCAGAGATTTCACCTGACT |
| GelEDown | GGTACTTTTATCGTAACTTACAC |
| SprEUp | CAATCGGTTGATGCAATCGGTG |
| SprEDown | GTACGAGCATTCGCAGTAAATTC |
| GelEprom | GAGAGAATTCGCTATGGTATTGAGTTATGAGG |
| Ef1091-5′ | GCTATGTTGGCTACTCAAGTG |
| Ef1091-3′ | TGTCCTGCAGGTGCTTTTAC |
| Ef0887-5′ | GAGAGAATTCGAAGGAATTGTCATTGTGCG |
| Ef0887-3′ | CAAAACGGATCCGACTCGC |
| Ef2490-5′ | GAGAGTCGACAGCAAAAGGGTTGTTGAAATA |
| Ef2490-3′ | CTCTGCATGCCCTACTTTCTCTGTTACTTAAT |
| Ef2488-5′ | GAGAGGATCCTAAACAGGGGAGTGTGTGACATG |
| Ef2488-3′ | GAGAGCATGCCAAACACCAAATGCATTATTTA |
| Ef2194-5′ | GGAACAACGCTAAACTTTTAC |
| Ef2194-3′ | CGCTCCTATTCTGCTGCTAA |
| OriF | CAATAATCGCATCCGATTGCA |
| SeqR | CCTATTATACCATATTTTGGAC |