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. 2008 Jun 6;190(16):5545–5554. doi: 10.1128/JB.01080-07

FIG. 6.

FIG. 6.

Transformation of compounds I and III by the TesR disruption (TesR−) mutant expressing each ORF. Cultures of the ORF9 disruption mutant (a to d) and the ORF8 disruption mutant (e to h) were used as reaction solutions after filter sterilization and dilution 10 times with 1/2 LB + 1/2 C medium. Each reaction solution was treated with the TesR− mutant carrying pMFY42 (negative control) (a, e), pMFYORF9 (b, f), pMFYORF8 (c, g), or pMFYORF9,8 (d, h) for 2 days, and the reaction solution was analyzed by HPLC 2 days after the start of the incubation. The TesR− strain is a mutant of TA441 with a mutation in TesR, a positive regulator of steroid degradation genes.