Figure 5.

BLM complements a phenotype of a sgs1 top3 S. cerevisiae strain. Yeast strain AMR61 (sgs1 top3) was transformed with plasmids pYES2, pSGS1, and pC4YES3 (normal BLM cDNA under GAL1 control), as well as with missense alleles of BLM under galactose control. Colonies were picked into microtiter wells, replica-plated onto minimal plates with galactose as the carbon source, and incubated at 30°C for 5 d. A comparison of the growth efficiency of these strains on increasing concentrations of hydroxyurea (HU) is shown. Each row displays six colonies from the transformation plates. Row A = +pYES2; row B = +pSGS1; row C = +pC4YES; row E = +pQ672R/BLM; row F = +pC1055S/BLM; row G = +pK695T/BLM.