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. 2008 Jun 16;76(9):4251–4258. doi: 10.1128/IAI.01570-07

FIG. 3.

FIG. 3.

Detection of lgt2 gene expression by RT-PCR. The RT-PCR was performed using the following nucleic acid templates: total RNA from strain 26404 was used for lanes 2, 4, 6 and 7; total RNA from mutant strain 26404lgt2 was used for lanes 9, 11, 13, and 14; chromosomal DNA from strain 26404 was used for lanes 1, 3, and 5; and chromosomal DNA from mutant strain 26404lgt2 was used for lanes 8, 10, and 12. Reaction sets contained the following primers: primers lgt587 and lgtR1071 were used for lanes 1, 2, 8, and 9; primers lgt1482 and lgtR2002 were used for lanes 3, 4, 10, and 11; and primers lgt2188 and lgtR3472 were used for lanes 5, 6, 12, and 13. The controls (lanes 7 and 14) used total RNA as the nucleic acid template without activation of the RT. GeneRuler DNA ladder mix (Fermentas) was used for the molecular size standards (in base pairs) (lane 15).