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Strain 26404lgt2 or 26404 whole-cell bacteria were incubated with preimmune or bactericidal anti-LOS rabbit serum for 1 h. The bacteria were incubated for another 1 h with fluorescein isothiocyanate-labeled goat anti-rabbit IgG secondary antibody. Samples were read on a Coulter XL-MCL flow cytometer, and data were analyzed with ADC 32 software.
