FIG. 3.

IFN-γ⁺ cells are detected by ICS in both spleen and liver quickly after LVS infection. C57BL/6J mice were infected with 10⁵ CFU of LVS i.d., and pooled single-cell suspensions of splenocytes from five naive and LVS-infected mice were prepared on the indicated days. (A) The percentages of IFN-γ⁺ cells in splenocytes and liver leukocytes were assessed by ICS and flow cytometry. (B) Representative dot plots after exclusion of aggregated cells (using forward light scatter patterns) for cells obtained from naive mice treated only with isotype control antibodies (left), cells obtained from naive mice stained with anti-CD45 and anti-IFN-γ (middle), and cells obtained from mice 5 days after LVS infection and stained with anti-CD45 and anti-IFN-γ (right), illustrating the gating strategy and patterns of IFN-γ⁺ cells. Results shown were derived from a single representative experiment of three total experiments of similar design.