FIG. 7.

Development of IFN-γ-producing myeloid cells after LVS vaccination does not depend on T or B lymphocytes. C57BL/6J (WT) or C57-RAG-1 mice were infected with 10⁵ CFU of LVS i.d. Single-cell suspensions of pooled splenocytes from five WT and three RAG-1 knockout naive and LVS-infected mice were prepared on day 4, and IFN-γ⁺ splenocytes (A) and liver leukocytes (B) were analyzed by ICS and flow cytometry. The total number of IFN-γ⁺ cells of each subpopulation, as defined by a panel of cell surface markers (see text), was derived by multiplying the percentage of IFN-γ⁺ cells by the number of total viable cells obtained. The absolute number of cells for each cell subpopulation, illustrated by sections in the stacked bars, is also provided in the legend on the right. Results shown were derived from a single representative experiment of three total experiments of similar design.