TABLE 1.
Evolution of cell types producing IFN-γ after i.d. LVS infectiona
| Cell type | % IFN-γ+ cells after LVS infection on day:
|
||||
|---|---|---|---|---|---|
| 0 | 1 | 3 | 5 | 7 | |
| Spleen cells | |||||
| NK cells | 1.0 | 2.4 | 21.0 | 15.0 | 2.5 |
| NK T cells | 2.6 | 2.0 | 8.0 | 8.0 | 3.3 |
| Macrophages | 0 | 0 | 1.9 | 4.3 | 1.1 |
| Polymorphonuclear leukocytes | 0 | 0 | 3.8 | 0.9 | 1.2 |
| DCs | 2.1 | 1.2 | 7.5 | 11.9 | 10.1 |
| T cells | 0.1 | 0.4 | 1.0 | 2.0 | 2.6 |
| Liver cells | |||||
| NK cells | 0.9 | 0.8 | 8.2 | 14.8 | 7.4 |
| NK T cells | 1.3 | 3.9 | 11.6 | 6.1 | 4.7 |
| Macrophages | 1.1 | 0.2 | 2.3 | 3.1 | 3.0 |
| Polymorphonuclear leukocytes | 0 | 1.9 | 2.1 | 3.5 | 3.2 |
| DCs | 1.3 | 2.2 | 9.7 | 14.9 | 9.6 |
| T cells | 0.2 | 0.3 | 2.9 | 3.9 | 8.5 |
a
Mice were infected with 105 CFU of LVS i.d. At the indicated time points after infection, mice were sacrificed, and splenic and liver leukocytes were prepared as pools from five individual mice and processed for determination of IFN-γ+ cells by ICS as described in Materials and Methods. The percentage of IFN-γ+ cells of each subtype, defined using multiparameter flow cytometry and a panel of markers as described in the text, is shown.