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Northern blot analysis of PRA1, PHR1, and control gene TEF2. (A) Gene expression when Candida strains SC5314 (wild type), RM1000 (parent of the pra1-deleted strain), and CAM35.1 (pra1 null) were grown in YPD at pH 6.0 (Y) or in IMDM at pH 7.4 (I). (B) Gene expression of the SC5314 Candida strain grown in D10 medium, in the absence (−Mφ) or presence (+Mφ) of RAW 264.7 macrophages, for the indicated times (in hours).
