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Colocalization of Pra1p-GFP and AF546-fibrinogen. Strains CAM38.3 (Pra1p-GFP expressing) and CAM35.1 (pra1 null) were grown overnight at 37°C in IMDM (without FBS [−FBS]) or I-10 (with FBS [+FBS]) and then stained with AF546-labeled fibrinogen as described in Materials and Methods and visualized at ×630 magnification. The arrow indicates colocalization.
