TABLE 2.
Oligonucleotides used in this study
| Name | Description | No. of nucleotides | Sequencea | Source or reference |
|---|---|---|---|---|
| oAM86 | PRA1 5′ UTR plus PstI | 35 | AAAACTGCAGAAGATGAGCTCGACTCTTCATCATC | This study |
| oAM87 | PRA1 3′ UTR | 23 | GTGACAAGTCGACCCAATCGGAC | This study |
| oAM92 | PRA1 end, GFP start forward | 53 | CATGCAGATGGTGAAGTCCACTGTATGAGTAAAGGAGAAGAACTTTTCACTGG | This study |
| oAM93 | PRA1 end, GFP start reverse | 53 | CCAGTGAAAAGTTCTTCTCCTTTACTCATACAGTGGACTTCACCATCTGCATG | This study |
| oAM94 | GFP end, PRA1 3′ UTR, forward | 61 | CACATGGCATGGATGAACTATACAAATAATTGTTAAGTTCAGGCATTAAACAATTTTTAAG | This study |
| oAM95 | GFP end, PRA1 3′ UTR, reverse | 61 | CTTAAAAATTGTTTAATGCCTGAACTTAACAATTATTTGTATAGTTCATCCATGCCATGTG | This study |
| oAM98 | PRA1 5′ UTR PCR cassette | 100 | CAACAATATCTCGTTGGAAAAGACCTTTGTTTGGTTAATCATTTTTTTTATTCACATCTATAATCACAAACTTTCTCTcgtaatacgactcactataggg | This study |
| oAM99 | PRA1 3′ UTR PCR cassette | 100 | TGCAATTAATCTTATTAATTCAAGCTATAAAAGATATCCATGAAACACCTTAAAAATTGTTTAATGCCTGAACTTAACaagggaacaaaagctgggtacc | This study |
| H1 | HIS1 forward | 20 | GGTACCTGGAGGATGAGGAG | C. Bachewich |
| H2 | HIS1 reverse | 25 | AATATTTATGAGAAACTATCACTTC | C. Bachewich |
| U1 | URA3 forward | 22 | TTGAAGGATTAAAACAGGGAGC | 17 |
| U2 | URA3 reverse | 24 | ATACCTTTTACCTTCAATATCTGG | 17 |
a
Underlining indicates the PstI site, boldface type indicates the ATG codon from the GFP sequence, and lowercase lettering indicates the URA or HIS cassette common sequence.