TABLE 3.
AF546-fibrinogen binding quantification in Candida cells in the absence or presence of unlabeled fibrinogen
| Strain (genotype) | AF546-fibrinogen binding (RFU)a
|
Binding inhibition (%)b | |
|---|---|---|---|
| Without unlabeled fibrinogen | With unlabeled fibrinogen | ||
| SC5314 (wild type) | 4,407 ± 879 | 1,174 ± 295 | 73.4 ± 10.0 |
| CAM35.1 (pra1/pra1) | 4,056 ± 512 | 1,323 ± 197 | 67.4 ± 7.9 |
a
Data were calculated from 10 individual Candida hyphal tips for each of 10 microscopic fields (100 cells) per condition and are expressed as relative fluorescence units (RFU) ± standard deviations.
b
The percentage of inhibition by unlabeled fibrinogen was calculated as [1 − (mean RFU of cell with unlabeled fibrinogen/mean RFU of cells without unlabeled fibrinogen)] × 100.