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. 2008 Jun 23;76(9):3881–3890. doi: 10.1128/IAI.01651-07

FIG. 6.

FIG. 6.

Listeria-specific T-cell response in IL-18ko/ko mice. Untreated C57BL/6 WT mice, IL-18ko/ko mice, and anti-IL-18 MAb-treated WT mice (WT+α-IL-18) were infected with 10,000 L. monocytogenes ova. (A) After 7 days of infection, total numbers of Listeria-specific CD8+ T cells per spleen were determined using SIINFEKL-restricted MHC class I tetramers. (B) Total numbers of IFN-γ- and TNF-α-producing CD8+/CD4+ T cells in the spleens of infected mice 7 days after infection. Cells were restimulated in vitro for 4 h with SIINFEKL peptide for CD8+ T cells and with LLO190-201 peptide for CD4+ T cells and subsequently stained for intracellular cytokines as described in Materials and Methods. Data are derived from three mice per group. (C) Five weeks after primary infection with 2,000 L. monocytogenes ova, C57BL/6 and IL-18ko/ko mice were reinfected with 200,000 L. monocytogenes ova. Depicted are total numbers of SIINFEKL tetramer-positive splenic CD8+ T cells 5 days after reinfection. (D) Total numbers of IFN-γ- and TNF-α-producing CD8+/CD4+ T cells in the spleens of mice after reinfection with 200,000 L. monocytogenes ova, determined as described for panel B. Data are shown for one out of two experiments with three mice per group.