TABLE 1.
Mouse group | Time postinfection (h) | Total no. (106) of cells in indicated splenic leukocyte subpopulationb
|
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---|---|---|---|---|---|---|---|
Macrophages | PMNsc (Gr1+) | DCs (CD11c+) | NK cells (DX5+) | B cells (B220+) | T cells (TCRβ+d) | ||
WT | 48 | 9.0 ± 1.4 | 1.7 ± 0.1 | 2.2 ± 0.3 | 6.4 ± 0.6 | 36.2 ± 5.7 | 23.3 ± 2.0 |
IL-18ko/ko | 12.5 ± 1.0 | 2.6 ± 0.3 | 2.9 ± 0.5 | 9.0 ± 0.6 | 51.8 ± 8.2 | 27.5 ± 3.4 | |
Control | 4.4 ± 1.3 | 0.8 ± 0.4 | 2.8 ± 1.0 | 3.7 ± 1.0 | 20.4 ± 7.0 | 11.0 ± 3.9 | |
α-IL-18 MAb treated | 7.9 ± 1.6 | 1.2 ± 0.1 | 3.5 ± 0.8 | 7.0 ± 1.4 | 27.6 ± 7.8 | 16.8 ± 4.2 | |
WT | 72 | 2.0 ± 0.4 | 0.7 ± 0.1 | 2.4 ± 0.4 | 3.4 ± 0.8 | 14.1 ± 2.5 | 7.2 ± 2.4 |
IL-18ko/ko | 6.6 ± 3.3 | 1.4 ± 0.3 | 4.2 ± 2.4 | 9.1 ± 2.2 | 26.3 ± 6.0 | 12.7 ± 4.9 | |
Control | 7.5 ± 3.1 | 0.7±0.2 | 4.0 ± 2.1 | 7.9 ± 2.8 | 19.9 ± 8.3 | 8.8 ± 2.7 | |
α-IL-18 MAb treated | 10.1 ± 1.4 | 3.2 ± 0.5 | 8.9 ± 1.2 | 8.1 ± 1.4 | 43.4 ± 3.8 | 25.2 ± 3.1 |
Mice were infected with 50,000 listeriae. Spleens of four mice per group were removed 48 and 72 h after infection. Total cell numbers were counted, and numbers of splenic leukocyte subpopulations were subsequently assessed by FACS analysis.
Numbers in bold indicate significant differences in total cell numbers between WT and IL-18ko/ko mice or control and antibody-treated mice (P < 0.05; Student's t test). Data are from one experiment representative of two.
PMNs, polymorphonuclear leukocytes.
TCRβ, T-cell receptor β.