TABLE 1.
Total cell numbers in spleens of WT versus IL-18ko/ko and control versus anti-IL-18 MAb-treated mice at early time points after L. monocytogenes infectiona
| Mouse group | Time postinfection (h) | Total no. (106) of cells in indicated splenic leukocyte subpopulationb
|
|||||
|---|---|---|---|---|---|---|---|
| Macrophages | PMNsc (Gr1+) | DCs (CD11c+) | NK cells (DX5+) | B cells (B220+) | T cells (TCRβ+d) | ||
| WT | 48 | 9.0 ± 1.4 | 1.7 ± 0.1 | 2.2 ± 0.3 | 6.4 ± 0.6 | 36.2 ± 5.7 | 23.3 ± 2.0 |
| IL-18ko/ko | 12.5 ± 1.0 | 2.6 ± 0.3 | 2.9 ± 0.5 | 9.0 ± 0.6 | 51.8 ± 8.2 | 27.5 ± 3.4 | |
| Control | 4.4 ± 1.3 | 0.8 ± 0.4 | 2.8 ± 1.0 | 3.7 ± 1.0 | 20.4 ± 7.0 | 11.0 ± 3.9 | |
| α-IL-18 MAb treated | 7.9 ± 1.6 | 1.2 ± 0.1 | 3.5 ± 0.8 | 7.0 ± 1.4 | 27.6 ± 7.8 | 16.8 ± 4.2 | |
| WT | 72 | 2.0 ± 0.4 | 0.7 ± 0.1 | 2.4 ± 0.4 | 3.4 ± 0.8 | 14.1 ± 2.5 | 7.2 ± 2.4 |
| IL-18ko/ko | 6.6 ± 3.3 | 1.4 ± 0.3 | 4.2 ± 2.4 | 9.1 ± 2.2 | 26.3 ± 6.0 | 12.7 ± 4.9 | |
| Control | 7.5 ± 3.1 | 0.7±0.2 | 4.0 ± 2.1 | 7.9 ± 2.8 | 19.9 ± 8.3 | 8.8 ± 2.7 | |
| α-IL-18 MAb treated | 10.1 ± 1.4 | 3.2 ± 0.5 | 8.9 ± 1.2 | 8.1 ± 1.4 | 43.4 ± 3.8 | 25.2 ± 3.1 | |
a
Mice were infected with 50,000 listeriae. Spleens of four mice per group were removed 48 and 72 h after infection. Total cell numbers were counted, and numbers of splenic leukocyte subpopulations were subsequently assessed by FACS analysis.
b
Numbers in bold indicate significant differences in total cell numbers between WT and IL-18ko/ko mice or control and antibody-treated mice (P < 0.05; Student's t test). Data are from one experiment representative of two.
c
PMNs, polymorphonuclear leukocytes.
d
TCRβ, T-cell receptor β.