FIG. 1.

Proteomic analysis of T3SS2-dependent secreted proteins. (A) SDS-PAGE of total secreted proteins from the POR-2 and POR-2 ΔvcrD2 strains. The gel was stained with Coomassie brilliant blue G-250. Protein bands that were excised and analyzed by N-terminal amino acid sequencing are indicated by arrows. The positions of molecular weight markers are indicated on the left. (B) Genomic organization of the VPA1362 (VopB2) and VPA1361 (VopD2) genes and sequence identities and similarities of the proteins with other putative T3SS translocon proteins.