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. 2008 Jun 9;76(9):4282–4289. doi: 10.1128/IAI.01738-07

FIG. 2.

FIG. 2.

VopB2 and VopD2 are specifically secreted via T3SS2. (A) Western blot analysis of bacterial pellets and supernatants of isogenic POR-1 strains. The lanes contained POR-1 (tdhAS deletion mutant) (lane 1), POR-2 (vcrD1 deletion mutant) (lane 2), POR-3 (vcrD2 deletion mutant) (lane 3), POR-2 ΔvopB2 (vcrD1 vopB2 mutant) (lane 4), and POR-2 ΔvopD2 (vcrD1 vopD2 mutant) (lane 5). Blots were probed with anti-VopB2 (αVopB2) (top), anti-VopD2 (αVopD2) (middle), and anti-VopP (αVopP) (bottom) antibodies. (B) Western blot analysis of supernatants of the POR-2 ΔvopB2 and POR-2 ΔvopD2 strains expressing VopB2 or VopD2. Blots were probed with anti-VopB2 (top) and anti-VopD2 (bottom) antibodies.