FIG. 6.

VopB2 and VopD2 are necessary for VopT translocation. Caco-2 cells were infected with V. parahaemolyticus mutant strains expressing VopT C-terminally fused with the catalytic domain of adenylate cyclase toxin, and the intracellular cAMP concentrations in infected cells were determined by an enzyme-linked immunosorbent assay. The error bars indicate the standard deviations for the results of triplicate experiments. An asterisk indicates that the results are significantly different from the results obtained with the parental strain (P < 0.01).