TABLE 1.

Serotype, sequence type, and ROS-inducing abilities of nine clinical S. pneumoniae isolates, D39 WT, and D39 AL- and PLY-deficient mutants

Strain	Serotype	STa	Reference or source	ROSmax (Mcpm)b
D39 WT	2	128	59	17 ± 5.3
D39 AL−	2	128	8	3.2 ± 0.8
D39 PLY−	2	128	11	2.1 ± 1.4
A17	14	124	Otitis media patient	20 ± 4.8
A6	19F	ND	Otitis media patient	13 ± 3.3
A7	9V	ND	Otitis media patient	11 ± 1.3
A22	6A	481	Otitis media patient	16 ± 5.8
A24	14	ND	Otitis media patient	19 ± 4.0
23261	11A	62	CCUG	17 ± 2.8
33774	5	ND	CCUG	12 ± 1.1
56	8	ND	44	1.8 ± 1.3
4496	1	3018	Women's and Children's Hospital, North Adelaide	4.0 ± 2.0

^aST, sequence type. ND, not determined.

^bValues are means ± standard deviations (three to five samples per group) for peak values of ROS produced by neutrophils in response to 50 μl of bacterial suspensions. (The suspensions contained 7 × 10⁸ bacteria/ml before autolysis.)