FIG. 3.

To confirm the presence and location of the T-cell epitopes in Rv3615c, the responses of constituent peptides from the Rv3615c pool were determined using an IFN-γ ELISPOT assay with PBMC isolated from M. bovis-infected cattle. The results are expressed in SFU per 10⁶ PBMC for each animal. The cattle used were animals 3420, 3450, and 3606.