TABLE 2.

Candidate antigens^a

M. tuberculosis H37Rv designation	M. bovis designation	% Amino acid sequence identity to M. tuberculosis H37Rv				High-expression conditionsb	Functionc
		M. tuberculosis	M. bovis	M. avium	M. bovis BCG Pasteur
Rv1211	Mb1243	100	100	70	100	Mtb	CHP
Rv1222	Mb1254	100	100	23	100	Mb	CHP
Rv1398c	Mb1433c	100	100		100	Mtb+Mb	CHP
Rv2081c	Mb2107c	100	100	13	74	Mb	Possible TP
Rv2876	Mb2901	100	99	6	98	Mb	Possible conserved TP
Rv3271c	Mb3299c	100	100		100	Mtb	Probable conserved IMP
Rv3407	Mb3441	100	100		100	Mtb	CHP
Rv3477	Mb3504	100	98	47	99	Mtb+Mb	PE family protein (PE31)
Rv3613c	Mb3643c	100	100		100	Mtb	HP
Rv3614c	Mb3644c	100	100		100	Mtb+Mb	CHP
Rv3615c	Mb3645c	100	100		100	Mtb+Mb	CHP
Rv3633	Mb3657	100	100	28	100	Mb	CHP
Rv3750c	Mb3776c	100	100		100	Mtb	Possible excisionase
Rv3866	Mb3896	100	100	13	100	Mb	CHP

^aThe 14 candidates were screened for stimulation of an IFN-γ response in M. bovis-infected cattle. Both the M. tuberculosis H37Rv and M. bovis AF2122/97 designations are indicated. The levels of amino acid homology of the M. tuberculosis protein to homologues in M. bovis and seven closely related species are indicated.

^bThe antigens were expressed in either four M. tuberculosis conditions (Mtb), two M. bovis conditions (Mb), or all six conditions (Mtb+Mb).

^cCHP, conserved hypothetical protein; HP, hypothetical protein; IMP, integral membrane protein; TP, transmembrane protein.