FIG. 5.

DraE⁺ E. coli is associated with caveolin and phosphorylated PI3K in bladder cells. The cells were infected with red fluorescent bacteria (red fluorescence in panels A, C, D, and F) expressing DraE [AAEC191A(pCC90-DraEstop, pUC-R-RFP)] for 2 h. After infection, the samples were fixed and immunofluorescently labeled with anti-caveolin antibodies (green fluorescence in panels B and C) or anti-p-PI3K antibodies (green fluorescence in panels E and F). Arrows indicate examples of bacteria colocalized with caveolin or p-PI3K.