FIG. 4.
(A) Mapping of the binding region on FH for YadA and Ail of Y. enterocolitica O:3. Strain YeO3 (SP YAOC), YeO3-Ail (SP Y-OC), YeO3-c-OCR (SP -A--2), and YeO3-c-Ail-OCR (SP ---) bacteria were incubated with truncated recombinant FH constructs representing SCRs 1-5, 1-6, 1-7, 8-11, 11-15, and 8-20. Following incubation, the bacteria were washed and whole-cell lysates were run in 12% SDS-PAGE and analyzed by immunoblotting with goat anti-FH and HRP-conjugated rabbit anti-goat antibodies. The factors expressed by the strains tested are marked as in Fig. 1. (B) Detection of recombinant FH constructs with goat anti-FH antiserum and HRP-conjugated rabbit anti-goat antibodies.