TABLE 1.
Bacterial strains, bacteriophages, and plasmids used in this work
| Bacterial strain, bacteriophage, or plasmid | Description | Source or reference |
|---|---|---|
| Y. enterocolitica strains | ||
| 6471/76 (YeO3) | Serotype O:3, patient isolate, wild type; SP YAOCa | 49 |
| YeO3-Ail | Δail::Km-GenBlock, Kmr; SP Y-OC | 7 |
| YeO3-Ail-R | Spontaneous rough derivative of YeO3-Ail, Kmr; SP Y--C | 7 |
| YeO3-Ail-OCR | Spontaneous OC mutant derivative of YeO3-Ail-R, Kmr; SP Y--- | 7 |
| YeO3-trs11 | Δ(wzx-wbcL)::Km-GenBlock, derivative of YeO3, Kmr; SP YAO-2 | 53 |
| YeO3-trs11-R | Spontaneous rough derivative of YeO3-trs11, Kmr; SP YA--2 | 53 |
| YeO3-OC | Δ(wzx-wbcQ), derivative of YeO3; SP YAO-1 | 7 |
| YeO3-OCR | Spontaneous rough derivative of YeO3-OC; SP YA--1 | 7 |
| YeO3-Ail-OC | Δail::Km-GenBlock, derivative of YeO3-OC, Kmr; SP Y-O- | 7 |
| YeO3-R2 | Spontaneous rough derivative of YeO3; SP YA-C | 3 |
| YeO3-028 | ΔyadA::Km-GenBlock, derivative of YeO3, Kmr; SP -AOC2 | 7 |
| YeO3-028-R1 | Spontaneous rough derivative of YeO3-O28, Kmr; SP -A-C1 | 7 |
| YeO3-028-OC | Δ(wzx-wbcQ) derivative of YeO3-028, Kmr; SP -AO-1 | 7 |
| 6471/76-c (YeO3-c) | Virulence plasmid cured derivative of YeO3; SP -AOC1 | 49 |
| YeO3-R1 | Spontaneous rough derivative of YeO3-c; SP -A-C2 | 3 |
| YeO3-c-OC | Δ(wzx-wbcQ) derivative of YeO3-c; SP -AO-3 | 7 |
| YeO3-c-OCR | Spontaneous rough derivative of YeO3-c-OC; SP -A--2 | 7 |
| YeO3-c-trs8 | Δ(wzx-wbcL)::Km-GenBlock, derivative of YeO3-c, Kmr; SP -AO-2 | 53 |
| YeO3-c-trs8R | Spontaneous rough derivative of YeO3-c-trs8, Kmr; SP -A--1 | 53 |
| YeO3-c-Ail | Δail::Km-GenBlock, derivative of YeO3-c, Kmr; SP --OC | 7 |
| YeO3-c-Ail-OC | Spontaneous OC mutant derivative of YeO3-c-Ail, Kmr; SP --O- | 7 |
| YeO3-c-Ail-R | Spontaneous rough derivative of YeO3-c-Ail, Kmr; SP ---C | 7 |
| YeO3-c-Ail-OCR | Spontaneous OC mutant derivative of YeO3-c-Ail-R, Kmr; SP ---- | 7 |
| E. coli JM103 | Sequencing host strain | 34 |
| Plasmids | ||
| pYMS4450 | Promoterless yadA cloned into pL2.1; yadA, Ampr | 50 |
| pL2.1 | pBR322 with the Ptac promoter, Ampr | 58 |
| Bacteriophages | ||
| φR1-37 | OC-specific phage of Y. enterocolitica O:3 | 53 |
| φR8-01 | Infects OAg- and OC-negative Y. enterocolitica O:3 bacteria grown at 22°C | This work |
a
YadA, Ail, OAg, and OC SPs.