FIG. 3.

rMP65 fluor uptake by activated MDM and DC. MDM (A) or DC (B) were left untreated or activated with LPS (1 μg/ml) or IFN-γ (100 ng/ml), alone or in combination, for 30 min at 37°C. The cells were incubated with 5 μg/ml of rMP65 fluor for 30 min or 6 h at 37°C. The rMP65 fluor uptake by different cell types is expressed as the MFI. Data are expressed as means ± SEM for four independent experiments. Statistical analysis was performed with ANOVA and corrected by the Bonferroni test for multiple comparisons. In the lower part of the figure, filled histograms represent the autofluorescence of cells, while the green lines represent fluorescence of rMP65 fluor uptake by cells for a 30-min incubation. Results of cytofluorimetric analysis presented are from one representative experiment.