FIG. 4.

Inhibition of clathrin-independent and clathrin-dependent uptake of rMP65 fluor. MDM or DC were left untreated or treated with nystatin (2.5 or 5 μg/ml) for 30 min at 4°C or with chlorpromazine (30 or 45 μM) for 30 min at 37°C. The cells were then stimulated for 2 h with 25 μg/ml of rMP65 fluor, transferrin fluor, and albumin fluor. Results are expressed as percentages of rMP65 fluor, albumin fluor, and transferrin fluor uptake. Data are expressed as means ± SEM for three independent experiments. *, P < 0.05 (nystatin- or chlorpromazine-treated cells versus untreated cells).