FIG. 5.

Colocalization of rMP65 with LAMP-1 and LAMP-2. DC were treated with rMP65 fluor (25 μg/ml) for 2 h at 37°C. The cells were then fixed, permeabilized, and incubated for 1 h with primary antibodies (mouse anti-human LAMP-1 and mouse anti-human LAMP-2). After incubation, the cells were treated for 30 min with secondary antibodies (anti-mouse immunoglobulin G conjugated with Cy). Cells were fixed and analyzed with a confocal microscope. The data reported are from one experiment out of three performed with similar results.