FIG. 11.

EspF-overexpressing E22 causes enrichment of ZO-1, ZO-2, and claudin into the pedestals and increases in pedestal lengths. RK13 cells expressing actin-GFP were infected with wild-type E22 transformed with pespF for 3 h. Infected cells expressing actin-GFP (A, E, and I) were fixed and immunostained by using anti-EspF (B, F, and J) and with anti ZO-1 (C), anti-ZO-2 (G), or anti-claudin-1 (K). Panel D is a merged image from panels A, B, and C; panel H is a merged image from panels E, F, and G; and panel L is a merged image from panels I, J, and K.