FIG. 8.

REPEC induces claudin rearrangement and recruitment to pedestals in RK13 cells. (A to F) Rearrangement of claudin by REPEC (E22). Uninfected RK13 cells expressing actin-GFP (A) were fixed and stained with anti-claudin (B), and both images were merged (C). RK13 cells expressing actin-GFP (D) were infected with red fluorescent E22 (F) and fixed and stained with anti-claudin (E), and the images were merged (F). (G to J) REPEC recruits claudin to the pedestals. The magnification of panel F shows the specific localization of actin-GFP pedestals (G) beneath the attached red bacteria (H), which are enriched with claudin (I). The merged images show the colocalization of actin-GFP pedestals and claudin.