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. 2008 Jun 30;76(9):4129–4136. doi: 10.1128/IAI.00334-08

FIG. 6.

FIG. 6.

Deletion of ibeT decreases type 1 fimbria expression by increasing the proportion of bacteria with the IE in the off orientation. (A) Five-microliter samples of exponential-phase cultures of strains BEN2908, BEN2908 ΔibeT, BEN2908 ΔibeA, and DM34 were spotted on a nitrocellulose membrane. Type 1 fimbriae were detected using an anti-type 1 fimbriae polyclonal antibody. *, spot intensity was significantly different (Mann-Whitney test; P < 0.01) from that of strain BEN2908. (B) The IE was PCR amplified with primers Ch1-F and Ch1-R from chromosomal DNAs of strains BEN2908, BEN2908 ΔibeT, and BEN2908 ΔibeA. The PCR products were digested by SnaBI, and restriction fragments were separated in 2% agarose gels. Std, DNA size standard.