FIG. 3.

Differential impairment of IL-17-dependent gene expression in male and female BALB/c-IL-17RA^KO mice. (A) Female, but not male, BALB/c-IL-17RA^KO mice show the impaired expression of CXC chemokines. Chemokine levels in sera from male and female IL-17RA^KO mice were determined by ELISA and are defined as the chemokine concentrations from P. gingivalis-infected mice as percentages of the levels of chemokines from sham-treated mice. (B) Female, but not male, B6-IL-17RA^KO mice show the impaired expression of CXC chemokines. Sera from B6-IL-17RA^KO mice were analyzed for Groα as described for panel A. (Groα data from the female B6 WT and IL-17RA^KO mice were modified from reference 66 with permission of the publisher.) (C) G-CSF expression is impaired in male, but not female, IL-17RA^KO mice. G-CSF serum levels in the indicated mice are shown, normalized as described for panel A. (D) Serum IL-6 production is not altered in IL-17RA^KO mice. IL-6 levels in serum from the indicated mice are shown, normalized as for panel A. (E) Serum TNF-α production is enhanced in male and female IL-17RA^KO mice. TNF-α levels in serum from the indicated mice are shown, normalized as for panel A. (F) Baseline levels of Groα are increased in IL-17RA^KO mice. Serum Groα levels from uninfected mice are shown. Standard deviations are shown, and significance was assessed by an unpaired t test. *, P < 0.05; n.s., not statistically different.