TABLE 1.
Nonfermentative gram-negative bacterial strains used in this study
| Species | No. of isolates | Origin/culture collection no.a | Identification methodb |
|---|---|---|---|
| P. aeruginosac | 2 | ATCC 9027, ATCC 27853 | |
| 15 | HNLP | ||
| BCCd | 1 | HST 8684 | RFLP with HaeIII/species-specific recA PCR |
| 1 | HC BC05 | RFLP with HaeIII/species-specific recA PCR | |
| B. cepacia | 1 | ATCC 25416 | RFLP with HaeIII |
| 3 | HST | RFLP with HaeIII/species-specific recA PCR | |
| 6 | HC | RFLP with HaeIII/species-specific recA PCR | |
| B. multivorans | 2 | LMG 13010, ATCC 17616 | RFLP with HaeIII |
| 1 | HST | RFLP with HaeIII/species-specific recA PCR | |
| B. cenocepacia lineage IIIA | 1 | LMG 18863 | RFLP with HaeIII |
| 37e | HNLP | RFLP with HaeIII/species-specific recA PCR | |
| 11e | HC | RFLP with HaeIII/species-specific recA PCR | |
| 31e | HST | RFLP with HaeIII/species-specific recA PCR | |
| B. cenocepacia lineage IIIB | 1 | LMG 16654 | RFLP with HaeIII |
| 2 | HC | RFLP with HaeIII/species-specific recA PCR | |
| 1 | LMG 18870 | RFLP with HaeIII | |
| B. stabilis | 3 | HC | RFLP with HaeIII/species-specific recA PCR |
| 1 | LMG 10929 | RFLP with HaeIII | |
| B. vietnamiensis | 1 | LMG 21820 | RFLP with HaeIII |
| B. dolosa | 1 | LMG 19467 | RFLP with HaeIII |
| B. ambifaria | 1 | LMG 20983 | RFLP with HaeIII |
| B. anthina | 1 | LMG 14191 | RFLP with HaeIII |
| B. pyrrocinia | 2 | HC Sm 20/06, HC Sm 66/06 | PCR for 16S rRNA |
| S. maltophilia | 11 | HC | |
| 13 | HNLP | ||
| 2 | HC Ax 247/06, HC Ax 335/06 | PCR for 16S rRNA | |
| A. xylosoxidans | 9 | HC | |
| 10 | HNLP | ||
| 1 | ATCC 19606 | ||
| Acinetobacter baumannii | 1 | HC Ab 54/06 | PCR for 16S rRNA |
| 9 | HNLP | ||
| Acinetobacter spp. | 1 | ATCC 27511 | |
| Ralstonia pickettii | 1 | HC Rp 127 | PCR for 16S rRNA |
ATCC, American Type Culture Collection, Manassas, VA; DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen; LMG, Laboratorium Microbiologie Gent Culture Collection, Ghent, Belgium; HNLP, Hospital de Niños La Plata, La Plata, Argentina; HST, Hospital Santa Trinidad, Córdoba, Argentina; HC, Hospital de Clínicas, Buenos Aires, Argentina.
All clinical isolates were identified by use of the API or the Vitek system and the nine additional phenotypic tests indicated in Materials and Methods (19). BCC clinical isolates were further identified by recA PCR-RFLP analysis with primers BCR1 and BCR2 and the HaeIII restriction enzyme, followed by recA PCR with species-specific primers (50). Some clinical isolates belonging to genera other than BCC were randomly chosen to confirm their identities by16S rRNA gene sequencing.
Only nonmucoid P. aeruginosa strains were included.
All BCC reference strains were kindly provided by Laura Galanternik, Hospital Gutierrez, Buenos Aires, Argentina.
One strain was identified as B. cenocepacia lineage IIIA type D by recA PCR-RFLP analysis with HaeIII, and the rest of the isolates were atypical strains identified as B. cenocepacia lineage IIIA but had a recA PCR-HaeIII RFLP profile not compatible with the ones previously described for B. cenocepacia (50).