TABLE 2.

Reproducibility levels obtained from cells before and after removing appendage structures calculated as D values for different spectral ranges^a

Species	Isolate	D values for the following spectral ranges (cm−1):
		Before shearing			After shearing and centrifugation
		3,000-2,800	1,500-1,300	1,200-900	3,000-2,800	1,500-1,300	1,200-900
B. cenocepacia	57 (HNLP)	2.13 ± 1.62	6.19 ± 5.30	25.0 ± 23.2	0.75 ± 0.43	0.93 ± 0.66	1.94 ± 1.07
	69 (HNLP)	2.71 ± 1.03	6.35 ± 5.29	30.1 ± 28.7	1.11 ± 1.07	1.82 ± 1.55	2.75 ± 0.66
	15 (HST)	1.94 ± 1.29	7.62 ± 6.64	13.2 ± 10.9	0.89 ± 068	0.95 ± 1.06	1.70 ± 0.88
P. aeruginosa	R2 (HST)	2.73 ± 1.96	8.36 ± 7.19	35.9 ± 40.2	1.20 ± 0.95	2.19 ± 1.07	3.43 ± 1.95
S. maltophilia	50428 (HST)	1.82 ± 1.69	10.12 ± 9.6	20.3 ± 23.2	0.31 ± 0.9	1.85 ± 1.34	2.99 ± 1.98
	35668 (HST)	1.9 ± 1.83	9.73 ± 8.25	15.3 ± 12.9	0.60 ± 0.40	0.96 ± 0.57	2.8 ± 2.1

^aD values were calculated (36) for the B. cenocepacia clinical isolates before and after shearing in the spectral regions 1,200 to 900 cm⁻¹ (assigned to carbohydrates), 1,500 to 1,300 cm⁻¹ (mixed region), and 3,000 to 2,800 cm⁻¹ (C—H stretching of >CH₂ and —CH₃). D values corresponding to one P. aeruginosa clinical isolate (isolate R2) and two S. maltophilia clinical isolates (isolates 50428 and 35668) are shown as examples of the typical D values obtained for genera other than Burkholderia. HNLP, Hospital de Niños La Plata, La Plata, Argentina; HST, Hospital Santa Trinidad, Córdoba, Argentina.