TABLE 1.

Detection of positive-strand HEV RNA as well as replicative negative-strand HEV RNA in nonliver tissues from chickens experimentally infected with avian HEV via a natural route of exposure

Tissue	No. of chickens positive for positive-strand HEV RNA (no. positive for replicative negative-strand HEV RNA)/total no. of chickens tested on the following dpi:
	1	3	5	7	10	16	20	24	28	35	42	56
Colorectum	1 (0)/2	1 (0)/2	2 (1)/2	1 (0)/2	2 (0)/2	1 (1)/2	2 (2)/2	1 (0)/2	0 (na)/2	2 (1)/2	0 (n)/2	2 (0)/4
Jejunum	0 (n)/2	0 (n)/2	0 (n)/2	1 (0)/2	2 (0)/2	1 (0)/2	2 (2)/2	0 (n)/2	0 (n)/2	2 (1)/2	0 (n)/2	0 (n)/4
Ileum	2 (0)/2	0 (n)/2	0 (n)/2	1 (1)/2	2 (2)/2	0 (n)/2	2 (1)/2	0 (n)/2	1 (0)/2	1 (1)/2	0 (n)/2	0 (n)/4
Duodenum	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	2 (1)/2	0 (n)/2	1 (0)/2	1 (0)/2	0 (n)/2	0 (n)/4
Cecum	1 (0)/2	0 (n)/2	0 (n)/2	1 (0)/2	2 (0)/2	2 (0)/2	2 (2)/2	1 (0)/2	2 (0)/2	2 (1)/2	1 (0)/2	0 (n)/4
Cecal tonsils	1 (0)/2	0 (n)/2	2 (0)/2	2 (0)/2	0 (n)/2	0 (n)/2	2 (0)/2	0 (n)/2	0 (n)/2	1 (1)/2	1 (0)/2	1 (1)/4
Thymus	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	1 (0)/2	0 (n)/2	0 (n)/4
Spleen	0 (n)/2	0 (n)/2	0 (n)/2	1 (0)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	1 (0)/2	0 (n)/2	0 (n)/4
Lung	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	1 (0)/2	1 (0)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	2 (0)/4
Heart	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	1 (0)/2	0 (n)/2	1 (0)/2	0 (n)/2	0 (n)/2	2 (0)/2	0 (n)/2	0 (n)/4
Kidney	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	1 (0)/2	1 (0)/2	0 (n)/2	0 (n)/4
Pancreas	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	0 (n)/2	1 (0)/2	0 (n)/2	0 (n)/4
Liverb	(0)/2	(0)/2	(0)/2	(0)/2	(0)/2	(1)/2	(2)/2	(1)/2	(0)/2	(1)/2	(0)/2	(0)/4

^an, not tested by the negative-strand-specific RT-PCR since they were negative for the positive-strand viral RNA by the regular RT-PCR.

^bThe results for positive-strand HEV RNA detection in liver samples were reported previously (2).