FIG. 1.

Relationship between experimentally detected and expected (theoretical) number of HHV-6 DNA copies in spiked serum samples. Series of coded samples were analyzed in a blinded fashion by different real-time PCR assays according to the protocols described in the Materials and Methods section. The estimated numbers of HHV-6 DNA copies obtained experimentally were plotted against the expected values. For samples containing 10 and 100 copies of spiked HHV-6, the mean copy number from two samples is presented.