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. 2008 Jun 25;46(8):2652–2658. doi: 10.1128/JCM.00192-08

FIG. 7.

FIG. 7.

Improved detection sensitivity by seminested RT-PCR and by genus subgroup primers with less degeneracy. (A) Seminested RT-PCR amplification of RNA extracted from 10-fold serial dilution of mumps virus stock using the pan-AVU-RUB-F1/AVU-RUB-R primer pair (1st run) and the pan-AVU-RUB-F2/AVU-RUB-R primer pair (2nd run). (B) Seminested RT-PCR amplification of RNA extracted from 10-fold serial dilution of mumps virus stock using the pan-PAR-F1/PAR-R primer pair (1st run) and the pan-PAR-F2/PAR-R primer pair (2nd run).