Effect of PMA on fluid phase endocytosis. Cells expressing CD4, K418, or H399 were incubated in BM containing HRP in the presence (filled symbols) or absence (open symbols) of 100 ng/ml PMA. At the end of the incubation period the cells were rapidly cooled to 4°C and washed extensively, and the cell-associated HRP was determined. □ and ▪, wt CD4; ○ and ●, K418; ▵ and ▴, H399.