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SPR analysis of CD4 peptide–adaptor complex binding. Biotinylated CD4 tail peptides were immobilized on an SA5 sensor surface and analyzed for binding of purified AP-2 and AP-1 adaptor complexes. The wt peptide bound both adaptor complexes with very low affinity (see Table 3); however, high-affinity adaptor binding was observed when peptides containing phosphorylated S408 were used.
