Table 1.

Oligonucleotides used for site-directed mutagenesis

Construct	Oligonucleotide
K418	5′-AGGGCACTGGCATCACTTCTTCTCACT
R412	5′-CTTCTCACTGAGTCATCTCTTGATCTG
H399	5′-CTTGGCGCCTTCAGTGCCGGCAC
K418 S408A	5′-GAGCGGATGGCACAGATCAAG
	and 5′-CTTGATCTGTGCCATCCGCTC
K418 S415A	5′-AGACTCCTCGCGAGAAGAAG
	and 5′-CTTCTTCTCTGCGAGGAGTCT
K418 S408A/ S415A	As for K418 S415A
K418 S408E	5′-TCTCTTGATCTGCTCCATCCGCTCTGC
K418 S415E	5′-CTTCTTCTCCTCGAGGAGTCTCTT
K418 S408E/ S415E	As for K418 S415E
K418 L413A/ L414A	5′-CTTCTTCTCACTGGCGGCTCTCTTGAT
K418 L413V	5′-CAGATCAAGCGAGTACTCAGTGAGAAGAAG
	and 5′-CTTCTTCTCACTGAGTACTCGCTTGATCTG
K418 L413V/	5′-CAGATCAAGAGAGTGGTCAGTGAGAAGAAG
L414V	and 5′-CTTCTTCTCACTGACCACTCTCTTGATCTG