FIG. 1.

Ribosome association profiles of S. enterica BipA under normal growth conditions. SB300A cells expressing minimal levels of BipA or BipA K18A were treated with 0.1 mg/ml chloramphenicol for 3 min prior to harvest. Cell lysates were prepared and separated over 7-to-47% sucrose gradients. Gradient fractions were TCA precipitated and analyzed for BipA by immunoblotting against the His₆ tag using a HisDetector Western blot kit. The positions of the 30S, 50S, and 70S ribosomal species are labeled, as well as those of polysomes.